國家衛生研究院 NHRI:Item 3990099045/10089
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 12145/12927 (94%)
造訪人次 : 908696      線上人數 : 1039
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧：
  • 您可在西文檢索詞彙前後加上"雙引號"，以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋，建議至進階搜尋限定作者欄位，可獲得較完整資料
    •  進階搜尋
    主頁 ‧ 登入 ‧ 上傳 ‧ 說明 ‧ 關於NHRI ‧ 管理 到手機版


    請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/10089


    題名: Development of dengue vaccine by using plga microparticle adsorbed with dengue subunit protein
    作者: Pan, CH;Hsieh, CH
    貢獻者: Division of Vaccine Research and Development
    摘要: Aims: Poly lactic-co-glycolic acid (PLGA) is a biocompatible and biodegradable material used in many biomedical applications, including bioactive agent delivery. In this study, we evaluated the immunogenicity of a particulate dengue subunit vaccine formulated by cationic PLGA microparticles adsorbed with recombinant dengue envelope protein domain III. Methods: Cationic PLGA microparticles were prepared by solvent evaporation in the water-phase containing a cationic surfactant-cetyltrimethyl ammonium bromide. The PLGA particulate dengue vaccine was formulated by incubation of cationic PLGA microparticles and recombinant dengue subunit protein. Results: The average size and zeta potential of PLGA particles were about 1 l m in diameter and + 22 mV. Mice were immunized by intramuscular injection three times with either PLGA formulated or soluble dengue vaccine. A significant increase of phagocytes and a pro-longed time for antigen release were detected in mice immunized with PLGA particulate dengue subunit vaccine. In addition, the specific IFN- c and IL-4 responses, as well as the anti-dengue IgG titers were significantly increased in mice immunized with PLGA particulate dengue subunit vaccine. Conclusions: In summary, the PLGA formulated particulate dengue subunit vaccine can enhance the immunogenicity of subunit vaccine by stimulating phagocytes to uptake antigen and depositing the antigen to extend the stimulation time.
    日期: 2016-10
    關聯: Basic and Clinical Pharmacology and Toxicology. 2016 Oct;119(Suppl. 2):35.
    Link to: http://dx.doi.org/10.1111/bcpt.12659
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1742-7835&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000385818700105
    顯示於類別:[潘建雄] 會議論文/會議摘要

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    ISI000385818700105.pdf46KbAdobe PDF424檢視/開啟


    在NHRI中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋