國家衛生研究院 NHRI:Item 3990099045/10090
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/10090


    Title: Preparation and characterization of a biodegradable polyurethane hydrogel and the hybrid gel with soy protein for 3D cell-laden bioprinting
    Authors: Lin, HH;Hsieh, FY;Tseng, CS;Hsu, SH
    Contributors: Institute of Cellular and Systems Medicine
    Abstract: 3D printing shows great potential for fabricating customized scaffolds for tissue regeneration. Using hydrogel as a bioink for cell printing provides a biological platform for basic research and potential medical treatments. In this study, a waterborne poly(e-caprolactone) (PCL)-based biodegradable polyurethane (PU) with a soft segment replaced with 20 mol% of poly(L-lactide) (PLLA) diol or poly (D, L-lactide) (PDLLA) diol was prepared. These two PUs formed compact packing structures at temperatures >= 37 degrees C. They responded differently to temperature changes and the presence of electrolytes because of the difference in the free volume. With their thermal-responsive properties, both PU dispersions could form a gel in 3 min with the gel modulus reaching about 6-8 kPa after 30 min. To enhance the structural integrity during layer-by-layer deposition, the hybrid hydrogel of PU and soy protein isolate (PU/SPI hybrid) was further developed. The PU/SPI hybrid dispersion could undergo rapid gelation at 37 degrees C with the modulus reaching 130 Pa in 1 min. Moreover, the PU/SPI hybrid gel was readily blended with cells and printed at 37 degrees C without preheating. Neural stem cells (NSCs) were embedded in the hydrogels and analyzed for cell viability, metabolism, proliferation, and gene expression of neural-related markers. Cells cultured in the PU/SPI hybrid construct had better survival and proliferation than those in the PU gel. The PU/SPI hybrid ink may provide unique rheological properties for direct cell/tissue printing at 37 degrees C and a biomimetic microenvironment for cell survival, growth, and differentiation.
    Date: 2016-11-07
    Relation: Journal of Materials Chemistry B. 2016 Nov 07;4(41):6694-6705.
    Link to: http://dx.doi.org/10.1039/c6tb01501h
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2050-750X&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000386336300008
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84992209641
    Appears in Collections:[Shan-Hui Hsu] Periodical Articles

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