國家衛生研究院 NHRI:Item 3990099045/1160
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    题名: Inhibition of SARS-CoV 3C-like protease activity by theaflavin-3,3 '- digallate (TF3)
    作者: Chen, CN;Lin, CPC;Huang, KK;Chen, WC;Hsieh, HP;Liang, PH;Hsu, JTA
    贡献者: Division of Biotechnology and Pharmaceutical Research
    摘要: SARS-CoV is the causative agent of severe acute respiratory syndrome (SARS). The virally encoded 3C-like protease (3CL(Pro)) has been presumed critical for the viral replication of SARS-CoV in infected host cells. In this study, we screened a natural product library consisting of 720 compounds for inhibitory activity against 3CLPro. Two compounds in the library were found to be inhibitive: tannic acid (IC50 = 3 mu M) and 3-isotheaflavin-3-gallate (TF2B) (IC50 = 7 mu M). These two compounds belong to a group of natural polyphenols found in tea. We further investigated the 3CL(Pro)-inhibitory activity of extracts from several different types of teas, including green tea, oolong tea, Puer tea and black tea. Our results indicated that extracts from Puer and black tea were more potent than that from green or oolong teas in their inhibitory activities against 3CLPro. Several other known compositions in teas were also evaluated for their activities in inhibiting 3CLPro. We found that caffeine, (-)-epigallocatechin gallte (EGCg), epicatechin (EC), theophylline (TP), catechin (C), epicatechin gallate (ECg) and epigallocatechin (EGC) did not inhibit 3CLPro activity. Only theaflavin-3,3'-digallate (TF3) was found to be a 3CLPro inhibitor. This study has resulted in the identification of new compounds that are effective 3CLPro inhibitors.
    关键词: Integrative & Complementary Medicine
    日期: 2005
    關聯: Evidence-based Complementary and Alternative Medicine. 2005;2:209-215.
    Link to: http://dx.doi.org/10.1093/ecam/neh081
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000232027400012
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=24344464802
    显示于类别:[徐祖安] 期刊論文
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