國家衛生研究院 NHRI:Item 3990099045/1164

NHRI > Institute of Biotechnology and Pharmaceutical Research > John Tsu-An Hsu > Periodical Articles > Item 3990099045/1164

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Title:	Stable RNA interference in Spodoptera frugiperda cells by a DNA vector-based method
Authors:	Lin, CC;Hsu, JTA;Huang, KL;Tang, HK;Lai, YK
Contributors:	Division of Biotechnology and Pharmaceutical Research
Abstract:	Double-stranded RNA (dsRNA)-mediated interference (RNAi) is a powerful tool for silencing of gene expression in many organisms. To establish a DNA vector-based method for stable RNAi in Spodoptera frugiperda cells (Sf9), we created a stably transfected Sf9 cell line to express large dsRNA fragment targeting to silence the firefly luciferase gene (luc). The luc dsRNA specifically and stably suppressed the baculovirus-mediated luciferase expression. Thus, gene silencing in Sf9 cells was achieved using DNA vectors similar to the facile design described in this study.
Keywords:	Biotechnology & Applied Microbiology
Date:	2006-02
Relation:	Biotechnology Letters. 2006 Feb;28(4):271-277.
Link to:	http://dx.doi.org/10.1007/s10529-005-5530-3
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0141-5492&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000236355000010
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33645285291
Appears in Collections:	[John Tsu-An Hsu] Periodical Articles

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