國家衛生研究院 NHRI:Item 3990099045/1164
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    题名: Stable RNA interference in Spodoptera frugiperda cells by a DNA vector-based method
    作者: Lin, CC;Hsu, JTA;Huang, KL;Tang, HK;Lai, YK
    贡献者: Division of Biotechnology and Pharmaceutical Research
    摘要: Double-stranded RNA (dsRNA)-mediated interference (RNAi) is a powerful tool for silencing of gene expression in many organisms. To establish a DNA vector-based method for stable RNAi in Spodoptera frugiperda cells (Sf9), we created a stably transfected Sf9 cell line to express large dsRNA fragment targeting to silence the firefly luciferase gene (luc). The luc dsRNA specifically and stably suppressed the baculovirus-mediated luciferase expression. Thus, gene silencing in Sf9 cells was achieved using DNA vectors similar to the facile design described in this study.
    关键词: Biotechnology & Applied Microbiology
    日期: 2006-02
    關聯: Biotechnology Letters. 2006 Feb;28(4):271-277.
    Link to: http://dx.doi.org/10.1007/s10529-005-5530-3
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0141-5492&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000236355000010
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33645285291
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