國家衛生研究院 NHRI:Item 3990099045/11726
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/11726


    Title: Interferon-stimulated gene 15 promotes lymph node metastasis via interacting Rac1 in oral squamous cell carcinoma cells
    Authors: Chen, YL;Wu, WL;Yen, YC;Wang, SH;Shen, YY;Chen, YW
    Contributors: National Institute of Cancer Research;Pathology Core Laboratory
    Abstract: Lymph node metastasis is the main factor of poor prognosis in oral squamous cell carcinoma (OSCC) patients. Using proteomic analysis, we found that interferon-stimulated gene 15 (ISG15) was the highest expressed protein related to lymph node metastasis. Abundant ISG15 was also observed in the microarray datasets and immunohistochemical of OSCC patients. In the orthotopic xenograft model, ISG15 knockdown decreased tumor lymphangiogenesis and lymph node metastasis. Similarly, ISG15 knockdown diminished cell migration, invasion and transendothelial migration in OSCC cells. Especially, ISG15-induced cell migration was in an intracellular ISGylation-independent manner and associated with membrane protrusions. Ectopic expression of ISG15 increased Rac1 activity and knockdown of Rac1 impaired ISG15-enhanced migration. Moreover, ISG15 was co-localized and interacted with Rac1 in the region of membrane protrusions by immunofluorescence and proximity ligation asays. Immunoprecipitated ISG15 interacted with Rac1, especially Rac1-GDP form. Our study indicated that ISG15 promotes cell migration and lymph node metastasis via regulation of Rac1 activity and physical interaction with Rac1.
    Date: 2018-12
    Relation: Cancer Science. 2018 Dec;109(Suppl. 2):214.
    Link to: https://doi.org/10.1111/cas.13904
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1347-9032&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000453773601023
    Appears in Collections:[Ying-ying Shen] Conference Papers/Meeting Abstract
    [Ya-Wen Chen] Conference Papers/Meeting Abstract

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