國家衛生研究院 NHRI:Item 3990099045/11773
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/11773


    Title: Convallatoxin enhance the ligand-induced mu-opioid receptor endocytosis and attenuate morphine antinociceptive tolerance in mice
    Authors: Chao, PK;Chang, HF;Ou, LC;Chuang, JY;Lee, PT;Chang, WT;Chen, SC;Ueng, SH;Hsu, JT;Tao, PL;Law, PY;Loh, HH;Yeh, SH
    Contributors: Institute of Biotechnology and Pharmaceutical Research;Center for Neuropsychiatric Research
    Abstract: Morphine is a unique opioid analgesic that activates the mu-opioid receptor (MOR) without efficiently promoting its endocytosis that may underlie side effects. Our objective was to discover a novel enhancer of ligand-induced MOR endocytosis and determine its effects on analgesia, tolerance and dependence. We used high-throughput screening to identify convallatoxin as an enhancer of ligand-induced MOR endocytosis with high potency and efficacy. Treatment of cells with convallatoxin enhanced morphine-induced MOR endocytosis through an adaptor protein 2 (AP2)/clathrin-dependent mechanism, attenuated morphine-induced phosphorylation of MOR, and diminished desensitization of membrane hyperpolarization. Furthermore, co-treatment with chronic convallatoxin reduced morphine tolerance in animal models of acute thermal pain and chronic inflammatory pain. Acute convallatoxin administration reversed morphine tolerance and dependence in morphine-tolerant mice. These findings suggest convallatoxin are potentially therapeutic for morphine side effects and open a new avenue to study MOR trafficking.
    Date: 2019-02-20
    Relation: Scientific Reports. 2019 Feb 20;9:Article number 2405.
    Link to: http://dx.doi.org/10.1038/s41598-019-39555-x
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2045-2322&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000459094800086
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85061780423
    Appears in Collections:[Shiu-Hwa Yeh] Periodical Articles
    [John Tsu-An Hsu] Periodical Articles
    [Shau-Hua Ueng] Periodical Articles
    [Pao-Luh Tao] Periodical Articles

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