國家衛生研究院 NHRI:Item 3990099045/1229
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 12145/12927 (94%)
造访人次 : 914820      在线人数 : 1396
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻
    主页 ‧ 登入 ‧ 上传 ‧ 说明 ‧ 关于NHRI ‧ 管理 到手机版


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/1229


    题名: Purification and characterization of human prolyl dipeptidase DPPS in Sf9 insect cells
    作者: Chen, YS;Chien, CH;Goparaju, CM;Hsu, JTA;Liang, PH;Chen, X
    贡献者: Division of Biotechnology and Pharmaceutical Research
    摘要: DPP8 is a new member of the prolyl dipeptidases, many of which have important biological functions in vivo. DPP8 catalyzes the cleavage at the carboxyl side of the proline residue at the penultimate position. To study its structure and biochemical properties, we have overexpressed the human DPP8 protein in baculovirus infected Sf9 cells. The protein is soluble and can be purified to homogeneity. Using the chromogenic H-Gly-Pro-pNA as the substrate, a kinetic study shows that purified DPP8 is active and has a similar k(cat) value as that of DPP-IV, a prolyl dipeptidase that is a drug target for type II diabetes. The kinetic constants of DPP8 are also determined for other chromogenic substrates, and the results indicate that DPP8 has substrate preference at both the P1 and P2 sites. The expression system provides means of better understanding the structure, catalytic mechanism, and biological function of DPP8 protein. (C) 2004 Elsevier Inc. All rights reserved.
    关键词: Biochemical Research Methods;Biochemistry & Molecular Biology;Biotechnology & Applied Microbiology
    日期: 2004-05
    關聯: Protein Expression and Purification. 2004 May;35(1):142-146.
    Link to: http://dx.doi.org/10.1016/j.pep.2003.12.019
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1046-5928&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000220617600019
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=10644284275
    显示于类别:[陳新(2002-2015)] 期刊論文
    [徐祖安] 期刊論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    000220617600019.pdf424KbAdobe PDF533检视/开启


    在NHRI中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈