國家衛生研究院 NHRI:Item 3990099045/1237
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    题名: Dipeptidyl peptidase 8/9-like activity in human leukocytes
    作者: Maes, MB;Dubois, V;Brandt, I;Lambeir, AM;Van der Veken, P;Augustyns, K;Cheng, JD;Chen, X;Scharpe, S;De Meester, I
    贡献者: Division of Biotechnology and Pharmaceutical Research
    摘要: The proline-specific dipeptidyl peptidases (DPPs) are emerging as a protease family with important roles in the regulation of signaling by peptide hormones. Inhibitors of DPPs have an intriguing, therapeutic potential, with clinical efficacy seen in patients with diabetes. Until now, only recombinant forms of DPP8 and DPP9 have been characterized. Their enzymatic activities have not been demonstrated in or purified from any natural source. Using several selective DPP inhibitors, we show that DPP activity, attributable to DPP8/9 is present in human PBMC. All leukocyte types tested (lymphocytes, monocytes, Jurkat, and U937 cells) were shown to contain similar DPP8/9-specific activities, and DPPII- and DPPIV-specific activities varied considerably. The results were confirmed by DPPIV/CD26 immunocapture experiments. Subcellular fractionation localized the preponderance of DPP8/9 activity to the cytosol and DPPIV in the membrane fractions. Using Jurkat cell cytosol as a source., a 30-fold, enriched DPP preparation was obtained, which had enzymatic characteristics closely related to the ones of DPP8 and/or -9, including inhibition by allo-Re-isoindoline and affinity for immobilized Lys-isoindoline.
    关键词: Cell Biology;Hematology;Immunology
    日期: 2007-05
    關聯: Journal of Leukocyte Biology. 2007 May;81(5):1252-1257.
    Link to: http://dx.doi.org/10.1189/jlb.0906546
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0741-5400&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000246121800013
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=34247898818
    显示于类别:[陳新(2002-2015)] 期刊論文

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