國家衛生研究院 NHRI:Item 3990099045/12508
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 12145/12927 (94%)
造訪人次 : 855265      線上人數 : 1048
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧：
  • 您可在西文檢索詞彙前後加上"雙引號"，以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋，建議至進階搜尋限定作者欄位，可獲得較完整資料
    •  進階搜尋
    主頁 ‧ 登入 ‧ 上傳 ‧ 說明 ‧ 關於NHRI ‧ 管理 到手機版


    請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/12508


    題名: Morphine produces potent antinociception, sedation, and hypothermia in humanized mice expressing human mu opioid receptor splice variants
    作者: Huang, YH;Wu, YW;Chuang, JY;Chang, YC;Chang, HF;Tao, PL;Loh, HH;Yeh, SH
    貢獻者: Institute of Biotechnology and Pharmaceutical Research;NHRI Graduate Student Program;Center for Neuropsychiatric Research
    摘要: Morphine is a strong painkiller acting through mu opioid receptor (MOR). Full-length 7-transmembrane (TM) variants of MOR share similar amino acid sequences of TM domains in rodents and humans; however, interspecies differences in N- and C-terminal amino acid sequences of MOR splice variants dramatically affect the downstream signaling. Thus, it is essential to develop a mouse model that expresses human MOR splice variants for opioid pharmacological studies. We generated two lines of fully humanized MOR mice (hMOR; mMOR mice), line #1 and #2. The novel murine model having human OPRM1 genes and human-specific variants was examined by reverse transcription polymerase chain reaction (RT-PCR) and the MinION nanopore sequencing. The differences in the regional distribution of MOR between wild-type (WT) and humanized MOR mice brains were detected by RNAscope and radioligand binding assay. hMOR; mMOR mice were characterized in vivo using a tail-flick, charcoal meal, open field, tail suspension, naloxone precipitation tests, and rectal temperature measurement. The data indicated that WT and humanized MOR mice exhibited different pharmacology of morphine, including antinociception, tolerance, sedation, and withdrawal syndromes, suggesting the presence of species difference between mouse and human MORs. Therefore, hMOR; mMOR mice could serve as a novel mouse model for pharmacogenetic studies of opioids.
    日期: 2020-06
    關聯: Pain. 2020 Jun;161(6):1177-1190.
    Link to: http://dx.doi.org/10.1097/j.pain.0000000000001823
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0304-3959&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000539257100007
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85084921513
    顯示於類別:[葉修華] 期刊論文
    [其他] 期刊論文
    [陶寶綠] 期刊論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    PUB32040076.pdf8016KbAdobe PDF270檢視/開啟


    在NHRI中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋