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http://ir.nhri.org.tw/handle/3990099045/13116
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| Title: | Betel nut arecoline induces different phases of growth arrest between normal and cancerous prostate cells through the reactive oxygen species pathway |
| Authors: | Shih, LJ;Wang, JY;Jheng, JY;Siao, AC;Lin, YY;Tsuei, YW;Kuo, YC;Chuu, CP;Kao, YH |
| Contributors: | Institute of Cellular and Systems Medicine |
| Abstract: | Prostate cancer (PCa) is a reproductive system cancer in elderly men. We investigated the effects of betel nut arecoline on the growth of normal and cancerous prostate cells. Normal RWPE‐ 1 prostate epithelial cells, androgen‐independent PC‐3 PCa cells, and androgen‐dependent LNCaP PCa cells were used. Arecoline inhibited their growth in dose‐ and time‐dependent manners. Arecoline caused RWPE‐1 and PC‐3 cell cycle arrest in the G2/M phase and LNCaP cell arrest in the G0/G1 phase. In RWPE‐1 cells, arecoline increased the expression of cyclin‐dependent kinase (CDK)‐1, p21, and cyclins B1 and D3, decreased the expression of CDK2, and had no effects on CDK4 and cyclin D1 expression. In PC‐3 cells, arecoline decreased CDK1, CDK2, CDK4, p21, p27, and cyclin D1 and D3 protein expression and increased cyclin B1 protein expression. In LNCaP cells, arecoline decreased CDK2, CDK4, and cyclin D1 expression; increased p21, p27, and cyclin D3 expression; had no effects on CDK1 and cyclin B1 expression. The antioxidant N‐acetylcysteine blocked the arecoline‐induced increase in reactive oxygen species production, decreased cell viability, altered the cell cycle, and changed the cell cycle regulatory protein levels. Thus, arecoline oxidant exerts differential effects on the cell cycle through modulations of regulatory proteins. |
| Date: | 2020-12-03 |
| Relation: | International Journal of Molecular Sciences. 2020 Dec 3;21(23):Article number 9219. |
| Link to: | http://dx.doi.org/10.3390/ijms21239219 |
| JIF/Ranking 2023: | http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1422-0067&DestApp=IC2JCR |
| Cited Times(WOS): | https://www.webofscience.com/wos/woscc/full-record/WOS:000597496300001 |
| Cited Times(Scopus): | https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85097093968 |
| Appears in Collections: | [褚志斌] 期刊論文
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| SCP85097093968.pdf | | 3209Kb | Adobe PDF | 351 | View/Open |
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