國家衛生研究院 NHRI:Item 3990099045/15484
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    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/15484


    Title: Protocol for efficient human MSC chondrogenesis via Wnt antagonism instead of TGF-beta
    Other Titles: Protocol for efficient human MSC chondrogenesis via Wnt antagonism instead of TGF-β
    Authors: Hsieh, CC;Chang, CC;Hsu, PJ;Chen, L;Yen, BL
    Contributors: Institute of Cellular and Systems Medicine
    Abstract: To better implement mesenchymal stem cell (MSC)-based therapy toward cartilage diseases, a more efficient and less off-target chondrogenesis protocol is needed. Here, we present a protocol to induce human MSC chondrogenesis via Wnt antagonism. We describe steps for pellet formation, Wnt antagonism-based chondrogenic induction, and refreshing the differentiation medium. We detail procedures for characterizing MSC chondrogenesis. By using Wnt antagonism instead of conventional transforming growth factor β-based induction, this protocol avoids the potential for induction of chondrocyte hypertrophy/osteogenesis or other lineages. For complete details on the use and execution of this protocol, please refer to Hsieh et al.
    Date: 2023-12-15
    Relation: STAR Protocols. 2023 Dec 15;4(4):Article number 102728.
    Link to: http://dx.doi.org/10.1016/j.xpro.2023.102728
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85176938954
    Appears in Collections:[Betty Lin-Ju Yen] Periodical Articles

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