國家衛生研究院 NHRI:Item 3990099045/16975

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Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/16975

Title:	Mechanism of malignant transformation in arecoline-exposed human oral keratinocytes
Authors:	Lai, TY;Ko, YC;Chen, YL;Lin, SF;Chuang, YJ;Shiah, SG
Contributors:	National Institute of Cancer Research
Abstract:	Areca nut chewing is associated with oral potentially malignant disorders (OPMDs), a spectrum of precancerous lesions with variable rates of malignant transformation (1.7–16.9%). This study investigates the molecular alterations underlying this process. Three immortalized oral keratinocyte lines were repeatedly exposed to arecoline, the primary alkaloid in areca nuts, for 5–8months. RNA-seq analysis and functional assays were conducted on vehicle control and arecoline-treated cell pairs. Two of the three cell lines transformed after arecoline exposure, exhibiting anchorage-independent growth in vitro and tumorigenicity in vivo. The upregulated pathways included reactive oxygen species metabolism, cell cycle progression, and regulation of stem cell. Increased expression of stemness-related genes was observed in clinical datasets including oral cancer and OPMDs. This change of gene expression suggests a potential association with epigenetic reprogramming, a recently identified hallmark of cancer. In summary, this study identifies potential molecular targets for intervention and highlights the role of epigenetic alterations in arecoline-induced malignant transformation.
Date:	2025-01-03
Relation:	Cancer Science. 2025 Jan 03;116(S1):462.
Link to:	http://dx.doi.org/10.1111/cas.16413
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1347-9032&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:001401044101122
Appears in Collections:	[Su-Fang Lin] Conference Papers/Meeting Abstract [Shine-Gwo Shiah] Conference Papers/Meeting Abstract

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