國家衛生研究院 NHRI:Item 3990099045/2288

NHRI > Institute of Molecular and Genomic Medicine > Shiou-Hwei Yeh(2001-2005) > Periodical Articles > Item 3990099045/2288

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2288

Title:	Detection of SARS-associated coronavirus in throat wash and saliva in early diagnosis
Authors:	Wang, WK;Chen, SY;Liu, IJ;Chen, YC;Chen, HL;Yang, CF;Chen, PJ;Yeh, SH;Kao, CL;Huang, LM;Hsueh, PR;Wang, JT;Sheng, WH;Fang, CT;Hung, CC;Hsieh, SM;Su, CP;Chiang, WC;Yang, JY;Lin, JH;Hsieh, SC;Hu, HP;Chiang, YP;Wang, JT;Yang, PC;Chang, SC
Contributors:	Division of Molecular and Genomic Medicine
Abstract:	The severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is thought to be transmitted primarily through dispersal of droplets, but little is known about the load of SARS-CoV in oral droplets. We examined oral specimens, including throat wash and saliva, and found large amounts of SARS-CoV RNA in both throat wash (9.58 x 10(2) to 5.93 x 10(6) copies/mL) and saliva (7.08 X 10(3) to 6.38 x 10(8) copies/mL) from all specimens of 17 consecutive probable SARS case-patients, supporting the possibility of transmission through oral droplets. Immunofluorescence study showed replication of SARS-CoV in the cells derived from throat wash, demonstrating the possibility of developing a convenient antigen detection assay. This finding, with the high detection rate a median of 4 days after disease onset and before the development of lung lesions in four patients, suggests that throat wash and saliva should be included in sample collection guidelines for SARS diagnosis.
Keywords:	Immunology;Infectious Diseases
Date:	2004-07
Relation:	Emerging Infectious Diseases. 2004 Jul;10(7):1213-1219.
Link to:	http://www.cdc.gov/ncidod/EID/vol10no7/03-1113.htm
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1080-6040&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000222456800005
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84984539674
Appears in Collections:	[Shiou-Hwei Yeh(2001-2005)] Periodical Articles

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