國家衛生研究院 NHRI:Item 3990099045/2498

NHRI > National Institute of Environmental Health Sciences > Louis W. Chang(1999-2009) > Periodical Articles > Item 3990099045/2498

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/2498

Title:	Clinical-scale high-throughput analysis of urinary 8-oxo-7,8-dihydro-2'-deoxyguanosine by isotope-dilution liquid chromatography-tandem mass spectrometry with on-line solid-phase extraction
Authors:	Hu, CW;Wang, CJ;Chang, LW;Chao, MR
Contributors:	Division of Environmental Health and Occupational Medicine
Abstract:	Background: Quantification of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo) in urine or blood is used to assess and monitor oxidative stress in patients. We describe the use of on-line solid-phase extraction (SPE) and isotope-dilution liquid chromatography-tandem mass spectrometry (LC-MS/MS) for automated measurement of urinary 8-oxodGuo. Methods: Automated purification of urine was accomplished with a switching valve and an Inertsil ODS-3 column. After the addition of N-15(5) labeled 8-oxodGuo as an internal standard, urine samples were analyzed within 10 min without sample purification. This method was applied to measure urinary 8-oxodGuo in a group of healthy persons (32 regular smokers and 35 nonsmokers). Urinary cotinine was also assayed by an isotope-dilution LC-MS/MS method. Results: The lower limit of detection was 5.7 ng/L on column (2.0 fmol). Inter- and intraday imprecision (CV) was < 5.0%. Mean recovery of 8-oxodGuo in urine was 99%-102%. Mean (SD) urinary concentrations of 8-oxodGuo in smokers [7.26 (3.14) mu g/g creatinine] were significantly higher than those in nonsmokers [4.69 (1.70) mu g/g creatinine; P < 0.005]. Urinary concentrations of 8-oxodGuo were significantly correlated with concentrations of cotinine in smokers (P < 0.05). Conclusions: This on-line SPE LC-MS/MS method is sufficiently sensitive, precise, and rapid to provide high-throughput direct analysis of urinary 8-oxodGuo without compromising quality and validation criteria. This method could be applicable for use in daily clinical practice for assessing oxidative stress in patients.
Keywords:	Medical Laboratory Technology
Date:	2006-07
Relation:	Clinical Chemistry. 2006 Jul;52(7):1381-1388.
Link to:	http://dx.doi.org/10.1373/clinchem.2005.063735
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0009-9147&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000238725800023
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=33745448772
Appears in Collections:	[Louis W. Chang(1999-2009)] Periodical Articles

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