國家衛生研究院 NHRI:Item 3990099045/2550

NHRI > National Institute of Environmental Health Sciences > Tsui-Chun Tsou > Periodical Articles > Item 3990099045/2550

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Title:	Arecoline inhibits the 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced cytochrome P450 1A1 activation in human hepatoma cells
Authors:	Chang, EE;Miao, ZF;Lee, WJ;Chao, HR;Li, LA;Wang, YF;Ko, YC;Tsai, FY;Yeh, SC;Tsou, TC
Contributors:	Division of Environmental Health and Occupational Medicine
Abstract:	In the present study, we investigated the effect of arecoline, a major areca nut alkaloid, on the 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD)induced activation of cytochrome P4501A1 (CYP1A1) in a human hepatoma cell line Huh-7. We treated Huh-7 cells with 10 nM TCDD in the presence of different concentrations of arecoline (50-300 mu M). Our results indicated that arecoline attenuated the TCDD-induced CYP1A1 enzyme activation with an inhibitory effect on cell proliferation. By using real-time RT-PCR, we demonstrated that arecoline inhibited the TCDD-induced activations of CYP1A1 and AhR repressor (AhRR) mRNA expression in a similar pattern. Our results revealed that arecoline inhibited AhR mRNA expression with no direct effect on CYP1A1 enzyme activity. Therefore, in our present study, the observed inhibitory effect of arecoline on CYP1A1 activation was not due to the up-regulation of AhRR or direct inhibitory effect on CYP1A1 Taken together, here we have demonstrated that arecoline attenuates the TCDD-induced CYP1A1 activation mainly via down-regulation of AhR expression in human hepatoma cells, suggesting the possible involvement of arecoline in the AhR-mediated metabolism of environmental toxicants in liver. (C) 2006 Elsevier B.V. All rights reserved.
Keywords:	Engineering, Environmental;Engineering, Civil;Environmental Sciences
Date:	2007-07-19
Relation:	Journal of Hazardous Materials. 2007 Jul;146(1-2):356-361.
Link to:	http://dx.doi.org/10.1016/j.jhazmat.2006.12.035
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0304-3894&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000248159700046
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=34250322706
Appears in Collections:	[Tsui-Chun Tsou] Periodical Articles [Lih-Ann Li] Periodical Articles [Ying-Chin Ko(2003-2009)] Periodical Articles

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