國家衛生研究院 NHRI:Item 3990099045/2688
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    題名: Profiling expression patterns and isolating differentially expressed genes by cDNA microarray system with colorimetry detection
    作者: Chen, JJW;Wu, R;Yang, PC;Huang, JY;Sher, YP;Han, MH;Kao, WC;Lee, PJ;Chiu, TF;Chang, F;Chu, YW;Wu, CW;Peck, K
    貢獻者: National Institute of Cancer Research
    摘要: A high-density cDNA microarray with colorimetry detection system to simultaneously monitor the expression of many genes on nylon membrane is described and characterized. To quantify the expression of genes and to isolate differentially expressed genes, the southern hybridization process on filter membranes was employed. The levels of gene expression were represented by color intensities generated by colorimetric reactions in place of hazardous radioisotopes or costly laser-induced fluorescence detection. The gene expression patterns on nylon membranes were digitized by devices such as an economical flatbed scanner or a digital camera. The quantitative information of gene expression was retrieved by image analysis software. Quantitative comparison of the northern dot-blotting method with the microarray system is described. Applications employing single-color detection as well as dual-color detection to isolate differentially expressed genes among thousands of genes are demonstrated. (C) 1998 Academic Press
    關鍵詞: Biotechnology & Applied Microbiology;Genetics & Heredity
    日期: 1998-08-01
    關聯: Genomics. 1998 Aug;51(3):313-324.
    Link to: http://dx.doi.org/10.1006/geno.1998.5354
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0888-7543&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000075674000001
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0032143452
    顯示於類別:[吳成文(1996-2008)] 期刊論文

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