國家衛生研究院 NHRI:Item 3990099045/3075
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    题名: Bioassay-guided purification and identification of PPARa/gamma agonists from Chlorella sorokiniana
    作者: Chou, YC;Prakash, E;Huang, CF;Lien, TW;Chen, X;Su, IJ;Chao, YS;Hsieh, HP;Hsu, JTA
    贡献者: Division of Biotechnology and Pharmaceutical Research;Division of Clinical Research
    摘要: This study isolated agonists of peroxisome proliferator activated receptors (PPARs) from the green algae Chlorella sorokiniana, using a bioassay-guided purification strategy. PPARs are widely recognized as the molecular drug targets for many diseases including hyperglycemia, diabetes, obesity and cancer. Two independent bioassays were developed. The first is the scintillation proximity assay, a ligand binding assay. The other is the cell-based transcriptional activation assay which uses the Dual-Luciferase (R) reporter system as the reporter gene under the control of the PPAR response element. Using these two assays, a PPAR gamma-active fraction, CE 3-3, was obtained from C. sorokiniana extracts, which was also able to activate PPAR alpha-mediated gene expression. To elucidate the active ingredients in the CE 3-3 fraction, GC-MS analysis was employed. The results showed that the CE 3-3 fraction consisted of at least ten fatty acids (FAs). The bioactivities of several of the individual FAs were evaluated for their PPAR gamma activity and the results showed that linolenic acid and linoleic acid were the most potent FAs tested. Our studies indicate that Chlorella sorokiniana could have potential health benefits through the dual activation of PPAR alpha/gamma via its unique FA constituents.
    关键词: Chemistry, Medicinal;Pharmacology & Pharmacy
    日期: 2008-05
    關聯: Phytotherapy Research. 2008 May;22(5):605-613.
    Link to: http://dx.doi.org/10.1002/ptr.2280
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0951-418X&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000256128000006
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=44149093347
    显示于类别:[徐祖安] 期刊論文
    [謝興邦] 期刊論文
    [趙宇生(2002-2013)] 期刊論文
    [陳新(2002-2015)] 期刊論文
    [蘇益仁(2002-2015)] 期刊論文

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