國家衛生研究院 NHRI:Item 3990099045/4553
English  |  正體中文  |  简体中文  |  Items with full text/Total items : 12145/12927 (94%)
Visitors : 908082      Online Users : 904
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
    •  Adv. Search
    Home ‧ Login ‧ Upload ‧ Help ‧ About ‧ Administer Goto mobile version


    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/4553


    Title: Gene therapy with tumor vaccine increases the survival of hepatoma- bearing mice
    Authors: Chao, Y;Shih, Y;Cheng, H;Wei, S;Chi, K;Yeh, S;Lee, S;Yang, W
    Contributors: National Institute of Cancer Research
    Abstract: Background. Tumor vaccines combined with cytokine gene therapy and Bacillus Calmette-Guerin (BCG) were tested for prevention and therapeutic effects in the H6 mouse hepatoma model. Methods. Plasmid DNA of expression vectors carrying cDNA of mouse interleukin (IL)-2 and mouse granulocyte- macrophage colony-stimulating factor (mGM-CSF) were used for transfection to obtain H6 mouse hepatoma cells that secreted IL-2 (H6/IL-2) or GM-CSF (H6/GM- CSF). For tumor prevention, groups of mice were immunized twice with irradiated tumor cells with untransduced H6, H6/IL-2, H6/GM-CSF, or an equal mixture of H6/IL-2 and H6/GM-CSF. Three weeks later, these mice were inoculated subcutaneously with live H6 hepatoma cells, and tumor growth was measured. For therapeutic studies, mice first inoculated with live H6 cells were treated three days later with various irradiated tumor cell vaccines alone or in combination with BCG. Subsequent tumor growth was measured. Results. In tumor prevention studies, significant protection from tumor growth has been observed in animals vaccinated with irradiated cytokine- secreting H6 cells compared with those immunized with irradiated parental H6 cells. In tumor therapy studies, subsequent administration of irradiated H6/GM-CSF cells in combination with BCG impeded the tumorigenicity of preinoculated live H6 hepatoma cells. Conclusions. These results suggest that cytokine-secreting tumor vaccines have a prophylactic effect and BCG, in combination with irradiated H6/GM-CSF cells, shows a synergistic effect on delaying the growth of H6 mouse hepatomas.
    Date: 1999
    Relation: Chinese Medical Journal (Taipei). 1999;62(10):682-689.
    Link to: http://www.ncbi.nlm.nih.gov/pubmed/10533297
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0032828641
    Appears in Collections:[Wen-Kuang Yang(1996-1999)] Periodical Articles

    Files in This Item:

    File Description SizeFormat
    SCP0032828641.pdf1865KbAdobe PDF607View/Open


    All items in NHRI are protected by copyright, with all rights reserved.

    Related Items in TAIR

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback