國家衛生研究院 NHRI:Item 3990099045/4553
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    题名: Gene therapy with tumor vaccine increases the survival of hepatoma- bearing mice
    作者: Chao, Y;Shih, Y;Cheng, H;Wei, S;Chi, K;Yeh, S;Lee, S;Yang, W
    贡献者: National Institute of Cancer Research
    摘要: Background. Tumor vaccines combined with cytokine gene therapy and Bacillus Calmette-Guerin (BCG) were tested for prevention and therapeutic effects in the H6 mouse hepatoma model. Methods. Plasmid DNA of expression vectors carrying cDNA of mouse interleukin (IL)-2 and mouse granulocyte- macrophage colony-stimulating factor (mGM-CSF) were used for transfection to obtain H6 mouse hepatoma cells that secreted IL-2 (H6/IL-2) or GM-CSF (H6/GM- CSF). For tumor prevention, groups of mice were immunized twice with irradiated tumor cells with untransduced H6, H6/IL-2, H6/GM-CSF, or an equal mixture of H6/IL-2 and H6/GM-CSF. Three weeks later, these mice were inoculated subcutaneously with live H6 hepatoma cells, and tumor growth was measured. For therapeutic studies, mice first inoculated with live H6 cells were treated three days later with various irradiated tumor cell vaccines alone or in combination with BCG. Subsequent tumor growth was measured. Results. In tumor prevention studies, significant protection from tumor growth has been observed in animals vaccinated with irradiated cytokine- secreting H6 cells compared with those immunized with irradiated parental H6 cells. In tumor therapy studies, subsequent administration of irradiated H6/GM-CSF cells in combination with BCG impeded the tumorigenicity of preinoculated live H6 hepatoma cells. Conclusions. These results suggest that cytokine-secreting tumor vaccines have a prophylactic effect and BCG, in combination with irradiated H6/GM-CSF cells, shows a synergistic effect on delaying the growth of H6 mouse hepatomas.
    日期: 1999
    關聯: Chinese Medical Journal (Taipei). 1999;62(10):682-689.
    Link to: http://www.ncbi.nlm.nih.gov/pubmed/10533297
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0032828641
    显示于类别:[楊文光(1996-1999)] 期刊論文

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