Acrylamide was reported present in high-temperature fried foods. Daily intake of foods may lead to various significant exposures. In vivo uptake of acrylamide may be subjected to conjugation with glutathione under the catalysis of glutathione transferase. This conjugated product can be excreted through urine and considered as a biomarker for acrylamide exposure. The objective of this study was to develop a highly specific, rapid, and sensitive method to quantitatively analyze urinary N-acetyl-S-(propionamide) cysteine (NASPC) to serve as a biomarker for the assessment of acryl-amide exposures. After mineral salt was precipitated with acetonitrile, urine samples were cleaned up using an automatic column-switching device coupled with a betasil diol cartridge as a solid-phase extraction column. The fraction containing NASPC was back-flushed on to a microbore betasil diol column for final chromatography and detected by a mass spectrometer, that consisted of an electrospray ion source and a triple-stage-quadrupole mass analyzer using multiple reaction monitoring (MRM) mode. The detection limit of this method was estimated to be lower than 0.02 ug/mL. The calibration curve was linear using human urine spiked at 0.05 ug/mL to 5.0 ug/mL. These results demonstrated the potential application for high-throughput and accurate determina-tion of N-acetyl-S-(propionamide) cysteine levels to assess exposure for future study on the acrylamide associated adverse health effects.
Date:
2004-08
Relation:
Drug Metabolism Reviews. 2004 Aug;36(Suppl. 1):41.
