國家衛生研究院 NHRI:Item 3990099045/5114

NHRI > National Institute of Infectious Diseases and Vaccinology > Shih-Jen Liu > Periodical Articles > Item 3990099045/5114

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/5114

Title:	Enhancement of specific cell-capture efficiency using a reversible dielectrophoresis field
Authors:	Shew, BY;Chu, HC;Chen, CK;Su, YC;Hsieh, YH;Lai, LJ;Liu, SJ;Leng, CH
Contributors:	Vaccine Research and Development Center
Abstract:	This work proposes using a periodic and reversible DEP (dielectrophoresis) field to improve the immuno-capture efficiency of the rare specific cells in a mixed cell suspension. The forward DEP field gently guides all the cells to interact with the bioprobes that are immobilized on a PVDF substrate in the reactor, and the reverse DEP field redistributes the un-bonded cells into the suspension for next reaction chance. The fluorescence visibility of the specific T-cells was also improved via quantum dots/tetramer complex staining. Accordingly, the specifically stained and captured cells could be imaged and counted. The experimental results indicated that the forward DEP can double the cell-capture efficiency compared with that without DEP enhancement, and the reversible DEP scheme can improve the efficiency about 25% further. This assay is also successively used to detect the population of rare cytotoxic Tc cells in a human blood sample; and the Tc cell has specific receptors on the membrane surface to identify the Epstein-Barr virus (EBV).
Date:	2010-09
Relation:	Sensors and Actuators, A: Physical. 2010 Sep;163(1):128-137
Link to:	http://dx.doi.org/10.1016/j.sna.2010.08.010
JIF/Ranking 2023:	http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0924-4247&DestApp=IC2JCR
Cited Times(WOS):	https://www.webofscience.com/wos/woscc/full-record/WOS:000283671000020
Cited Times(Scopus):	http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=77957686858
Appears in Collections:	[Shih-Jen Liu] Periodical Articles [Chih-Hsiang Leng] Periodical Articles

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