國家衛生研究院 NHRI:Item 3990099045/5114
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    題名: Enhancement of specific cell-capture efficiency using a reversible dielectrophoresis field
    作者: Shew, BY;Chu, HC;Chen, CK;Su, YC;Hsieh, YH;Lai, LJ;Liu, SJ;Leng, CH
    貢獻者: Vaccine Research and Development Center
    摘要: This work proposes using a periodic and reversible DEP (dielectrophoresis) field to improve the immuno-capture efficiency of the rare specific cells in a mixed cell suspension. The forward DEP field gently guides all the cells to interact with the bioprobes that are immobilized on a PVDF substrate in the reactor, and the reverse DEP field redistributes the un-bonded cells into the suspension for next reaction chance. The fluorescence visibility of the specific T-cells was also improved via quantum dots/tetramer complex staining. Accordingly, the specifically stained and captured cells could be imaged and counted. The experimental results indicated that the forward DEP can double the cell-capture efficiency compared with that without DEP enhancement, and the reversible DEP scheme can improve the efficiency about 25% further. This assay is also successively used to detect the population of rare cytotoxic Tc cells in a human blood sample; and the Tc cell has specific receptors on the membrane surface to identify the Epstein-Barr virus (EBV).
    日期: 2010-09
    關聯: Sensors and Actuators, A: Physical. 2010 Sep;163(1):128-137
    Link to: http://dx.doi.org/10.1016/j.sna.2010.08.010
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0924-4247&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000283671000020
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=77957686858
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