國家衛生研究院 NHRI:Item 3990099045/5721
English  |  正體中文  |  简体中文  |  Items with full text/Total items : 12145/12927 (94%)
Visitors : 910401      Online Users : 780
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
    •  Adv. Search
    Home ‧ Login ‧ Upload ‧ Help ‧ About ‧ Administer Goto mobile version


    Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/5721


    Title: Rapid and sensitive detection of cancer cells by coupling with quantum dots and immunomagnetic separation at low concentrations
    Authors: Hsieh, YH;Lai, LJ;Liu, SJ;Liang, KS
    Contributors: Division of Vaccine Research and Development
    Abstract: This work presents a rapid and sensitive method for detecting cancer cells at low concentration. In this method, two biomarkers of T-help cancer cells are detected simultaneously. One biomarker is conjugated with magnetic beads to separate T-help cell from the mixed cells and the other biomarker, associated with quantum dots, is used to detect fluorescence. The specific T-help cells can be quantified using the relationship between the QD fluorescence intensity and the cell frequency following magnetic separation. The intensity of fluorescence increases linearly with the frequency of T-help cells from 10-7 to 10-3, and neither B cells nor red blood cells interfere with the detection of T-help cells. Moreover, the total detection time is under 15 min, even though the frequency of specific T-help cells is as low as 5 ? 10-7. The numerous advantages of detecting specific cells at low concentration using the presented method include ease of preparation, low cost, fast detection, and high sensitivity.
    Date: 2011-06-15
    Relation: Biosensors and Bioelectronics. 2011 Jun 15;26(10):4249-4252.
    Link to: http://dx.doi.org/10.1016/j.bios.2011.04.023
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=0956-5663&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000291904000045
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=79956355430
    Appears in Collections:[Shih-Jen Liu] Periodical Articles

    Files in This Item:

    File Description SizeFormat
    PB2011052414.pdf354KbAdobe PDF431View/Open


    All items in NHRI are protected by copyright, with all rights reserved.

    Related Items in TAIR

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback