國家衛生研究院 NHRI:Item 3990099045/5906
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 12145/12927 (94%)
造访人次 : 906215      在线人数 : 800
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻
    主页 ‧ 登入 ‧ 上传 ‧ 说明 ‧ 关于NHRI ‧ 管理 到手机版


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/5906


    题名: Colloid-based multiplexed screening for plant biomass-degrading glycoside hydrolase activities in microbial communities
    作者: Reindl, W;Deng, K;Gladden, JM;Cheng, G;Wong, A;Singer, SW;Singh, S;Lee, JC;Yao, CH;Hazen, TC;Singh, AK;Simmons, BA;Adams, PD;Northen, TR
    贡献者: Institute of Biotechnology and Pharmaceutical Research
    摘要: The enzymatic hydrolysis of polysaccharides into fermentable sugars is a crucial step in the conversion of biomass to lignocellulosic biofuels. An efficient hydrolysis is highly dependent on the identification and characterization of optimal glycoside hydrolases. However, existing techniques for characterizing activity are limited by the range of reaction conditions that can be used, sample complexity, and throughput. The method we present is a multiplexed approach based on nanostructure-initiator mass spectrometry (NIMS) that allows for the rapid analysis of several glycolytic activities in parallel under diverse assay conditions. By forming colloids, it was possible to perform aqueous reactions in tubes and microwell plates despite the substrate analogs' hydrophobic perfluorinated tags. This method was validated by analyzing standard enzymatic parameters (temperature, pH, and kinetics) of beta-glucosidase and beta-xylosidase in separate setups. The multiplexity of this assay system was demonstrated by the simultaneous analysis of beta-glucosidase and beta-xylosidase activities, which was then used to profile environmental samples. Enzymes secreted by microbial communities within these samples were extracted by washing the sample with buffer. Enzymatic activities were directly detected within this crude extract without any further sample pretreatment steps. The multiplexed analysis of beta-glucosidase, exo-/endoglucanase, and xylanase activities was applied for a detailed characterization of previously unknownglycoside hydrolase activities. The results show the suitability of the described method for the rapid screening of crude environmental samples under a wide range of conditions to determine enzyme activities from the microbial communities present within these samples.
    日期: 2011-08
    關聯: Energy and Environmental Science. 2011 Aug;4(8):2884-2893.
    Link to: http://dx.doi.org/10.1039/c1ee01112j
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1754-5692&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000293213600034
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=79960999883
    显示于类别:[李靜琪] 期刊論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    ISI000293213600034.pdf456KbAdobe PDF752检视/开启


    在NHRI中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈