Introduction: Congenital or acquired bone defects are major problems in orthopedic surgery. In this study, we conjugated a hydrophobic polyester compound, BOX, into the PLGA/PEG thermal hydrogel for control release model. In the past few years, we had already known that BMP-2 plays an important role in bone formation. The aim of this study, we created a model of critical-sized detect and design implants with osteoinductive growth factor for bone remodeling. Materials and Methods: 45 female Wistar rats were assigned into five groups (n = 9), and created a 5-mm segmental bone defect on bilateral ulnas. Through this study, we can validate the efficiency of collagen sponges and BOX hydrogel cooperating with rhBMP-2 on the critical-sized defect healing. Results: Through the data, we demonstrated the efficiency of Collagen/BOX implants combined with BMP-2 has better osteoinductive effect and can promote fracture healing on critical-sized bone defect model. Among HE staining of bone defect sections in these two experimental groups at 4 weeks after surgery demonstrated that there were regenerated bone elements formation, such as endochondral cells, osteoblasts and osteocytes. We found that bone formation at Collagen/BOX group combined with the vascular penetration of new blood vessels into the redeveloped area.
Date:
2012-09
Relation:
Journal of Tissue Engineering and Regenerative Medicine. 2012 Sep;6(Suppl. 1):19-20.
