An implantable and dual functional needle-type microprobe (500 μm o.d.) composed of a microdialysis probe and 13 optical fibers was designed and constructed (combomicroprobe). This combo-microprobe is capable of in vivo neurochemical delivery/analysis and photoenergy transmission /detection. The combo-microprobe was implanted into anesthetized rat brains and was used to simultaneously observe the cerebral ischemiainduced increase in extracellular glutamate concentration, which was performed by the microdialysis perfusion and analysis; and the increase in blood brain barrier permeability, which was performed by the optical fibers to monitor the extravasated fluorescent nanospheres. This combo-microprobe can be applied to various in vivo biomedical investigations in the future.
Date:
2005-10
Relation:
9th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2005. 2005 Oct;1:1032-1034.
