國家衛生研究院 NHRI:Item 3990099045/8994

NHRI > Institute of Molecular and Genomic Medicine > Jyh-Lyh Juang > Patents > Item 3990099045/8994

Please use this identifier to cite or link to this item: http://ir.nhri.org.tw/handle/3990099045/8994

Title:	Baculovirus-based expression system
Authors:	Juang, JL;Lee, DF
Contributors:	Institute of Molecular and Genomic Medicine
Abstract:	Compositions and methods are provided that relate to a recombinant virus-based vector, e.g., a baculovirus-based vector, that allows the expression of an exogenous target protein in non-permissive cells (e.g., non-permissive insect cells or mammalian cells) in the absence of expression of a detectable selection marker. The vector includes a nucleic acid sequence encoding a detectable selection marker which is controlled by a promoter that is active in host cells used to screen for recombinant virus but is silent in the non-permissive cell used for expression of the exogenous target protein. The vector also includes an exogenous nucleic acid sequence encoding a target protein under the control of a promoter that is active in the non-permissive cell. This system allows the selection marker to be expressed during viral plaque screening, but not while the target protein is being produced.
Date:	2004-11-09
Relation:	US6814963B2/2004-11-09
Link to:	http://worldwide.espacenet.com/publicationDetails/biblio?CC=US&NR=6814963&KC=B2
Appears in Collections:	[Jyh-Lyh Juang] Patents

Files in This Item:

There are no files associated with this item.