國家衛生研究院 NHRI:Item 3990099045/8994
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    題名: Baculovirus-based expression system
    作者: Juang, JL;Lee, DF
    貢獻者: Institute of Molecular and Genomic Medicine
    摘要: Compositions and methods are provided that relate to a recombinant virus-based vector, e.g., a baculovirus-based vector, that allows the expression of an exogenous target protein in non-permissive cells (e.g., non-permissive insect cells or mammalian cells) in the absence of expression of a detectable selection marker. The vector includes a nucleic acid sequence encoding a detectable selection marker which is controlled by a promoter that is active in host cells used to screen for recombinant virus but is silent in the non-permissive cell used for expression of the exogenous target protein. The vector also includes an exogenous nucleic acid sequence encoding a target protein under the control of a promoter that is active in the non-permissive cell. This system allows the selection marker to be expressed during viral plaque screening, but not while the target protein is being produced.
    日期: 2004-11-09
    關聯: US6814963B2/2004-11-09
    Link to: http://worldwide.espacenet.com/publicationDetails/biblio?CC=US&NR=6814963&KC=B2
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