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    請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/16710


    題名: Novel naphthyridones targeting pannexin 1 for colitis management
    作者: Hsueh, WY;Wu, YL;Weng, MT;Liu, SY;Santavanond, JP;Liu, YC;Lin, C;Lai, CN;Lu, YR;Hsu, JY;Gao, HY;Lee, JC;Wei, SC;Lyu, PC;Poon, IKH;Hsieh, HP;Chiu, YH
    貢獻者: Institute of Biotechnology and Pharmaceutical Research;Institute of Population Health Sciences
    摘要: Pannexin 1 (PANX1) forms cell-surface channels capable of releasing signaling metabolites for diverse patho-physiological processes. While inhibiting dysregulated PANX1 has been proposed as a therapeutic strategy for many pathological conditions, including inflammatory bowel disease (IBD), low efficacy, or poor specificity of classical PANX1 inhibitors introduces uncertainty for their applications in basic and translational research. Here, hit-to-lead optimization is performed and a naphthyridone, compound 12, is identified as a new PANX1 inhibitor with an IC50 of 0.73 mu m that does not affect pannexin-homologous LRRC8/SWELL1 channels. Using structure-activity relationship analysis, mutagenesis, cell thermal shift assays, and molecular docking, it is revealed that compound 12 directly engages PANX1 Trp74 residue. Using a dextran sodium sulfate mouse model of IBD, it is found that compound 12 markedly reduced colitis severity, highlighting new PANX1 inhibitors as a proof-of-concept treatment for IBD. These data describe the mechanism of action for a new PANX1 inhibitor, uncover the binding site for future drug design, and present a targeted strategy for treating IBD.
    日期: 2024-12-30
    關聯: Advanced Science. 2024 Dec 30;Article in Press.
    Link to: http://dx.doi.org/10.1002/advs.202411538
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=2198-3844&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:001388330600001
    Cited Times(Scopus): https://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=85213705839
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