國家衛生研究院 NHRI:Item 3990099045/6820
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 12145/12927 (94%)
造访人次 : 911558      在线人数 : 894
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻
    主页 ‧ 登入 ‧ 上传 ‧ 说明 ‧ 关于NHRI ‧ 管理 到手机版


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: http://ir.nhri.org.tw/handle/3990099045/6820


    题名: H2O2 accumulation mediates differentiation capacity alteration, but not proliferative decline in senescent human fetal mesenchymal stem cells
    作者: Ho, PJ;Yen, ML;Tang, BC;Chen, CT;Yen, BL
    贡献者: Institute of Cellular and Systems Medicine;Institute of Biotechnology and Pharmaceutical Research
    摘要: Aims: Mesenchymal stem cells (MSCs) with multilineage differentiation capacity and immunomodulatory properties are novel sources for cell therapy. However, in vitro expansion of these rare somatic stem cells leads to senescence, resulting in declines of differentiation and proliferative capacities. We therefore investigated the mechanisms mediating senescence in human fetal MSC termed placenta-derived multipotent cells (PDMCs). Results: Long-term cultured PDMCs underwent senescence, with increased levels of H2O2—a reactive oxygen species (ROS), positive -galactosidase staining, decreased SIRT1 expression, increased p21 expression, and cell cycle arrest at G0/G1 phase. Senescent PDMCs also showed decreased osteogenic capacity. Mechanistically, increased p21 expression and proliferative decline were not due to elevated H2O2 levels nor mediated by p53. Instead, inhibition of protein kinase C (PKC)-and -in senescent PDMCs decreased p21 expression and reversed cell cycle arrest. H2O2 was involved in the alteration of differentiation potential since scavenging of H2O2 restored expression of c-MAF, an osteogenic and age-sensitive transcription factor, and osteogenesis capacity in senescent PDMCs. Innovation: Our findings not only show the effects of senescence on MSCs, but also reveal mechanisms involved in mediating decreased proliferation and differentiation capacity. Moreover, targeting increased levels of H2O2 associated with senescence may reverse the decreased osteogenesis capacity of senescent MSCs. Conclusion: Our study suggests that the two biological consequences of senescence, differentiation alteration and proliferative decline, in fetal MSCs are distinctly regulated by H2O2-c-MAF and PKC-p21 pathways, respectively.
    日期: 2013-05
    關聯: Antioxidants and Redox Signaling. 2013 May;18(15):1895-1905.
    Link to: http://dx.doi.org/10.1089/ars.2012.4692
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1523-0864&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000317478500001
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84876104810
    显示于类别:[陳炯東] 期刊論文
    [顏伶汝] 期刊論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    PUB23088254.pdf1259KbAdobe PDF641检视/开启


    在NHRI中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈