國家衛生研究院 NHRI:Item 3990099045/6820
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    題名: H2O2 accumulation mediates differentiation capacity alteration, but not proliferative decline in senescent human fetal mesenchymal stem cells
    作者: Ho, PJ;Yen, ML;Tang, BC;Chen, CT;Yen, BL
    貢獻者: Institute of Cellular and Systems Medicine;Institute of Biotechnology and Pharmaceutical Research
    摘要: Aims: Mesenchymal stem cells (MSCs) with multilineage differentiation capacity and immunomodulatory properties are novel sources for cell therapy. However, in vitro expansion of these rare somatic stem cells leads to senescence, resulting in declines of differentiation and proliferative capacities. We therefore investigated the mechanisms mediating senescence in human fetal MSC termed placenta-derived multipotent cells (PDMCs). Results: Long-term cultured PDMCs underwent senescence, with increased levels of H2O2—a reactive oxygen species (ROS), positive -galactosidase staining, decreased SIRT1 expression, increased p21 expression, and cell cycle arrest at G0/G1 phase. Senescent PDMCs also showed decreased osteogenic capacity. Mechanistically, increased p21 expression and proliferative decline were not due to elevated H2O2 levels nor mediated by p53. Instead, inhibition of protein kinase C (PKC)-and -in senescent PDMCs decreased p21 expression and reversed cell cycle arrest. H2O2 was involved in the alteration of differentiation potential since scavenging of H2O2 restored expression of c-MAF, an osteogenic and age-sensitive transcription factor, and osteogenesis capacity in senescent PDMCs. Innovation: Our findings not only show the effects of senescence on MSCs, but also reveal mechanisms involved in mediating decreased proliferation and differentiation capacity. Moreover, targeting increased levels of H2O2 associated with senescence may reverse the decreased osteogenesis capacity of senescent MSCs. Conclusion: Our study suggests that the two biological consequences of senescence, differentiation alteration and proliferative decline, in fetal MSCs are distinctly regulated by H2O2-c-MAF and PKC-p21 pathways, respectively.
    日期: 2013-05
    關聯: Antioxidants and Redox Signaling. 2013 May;18(15):1895-1905.
    Link to: http://dx.doi.org/10.1089/ars.2012.4692
    JIF/Ranking 2023: http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcAuth=NHRI&SrcApp=NHRI_IR&KeyISSN=1523-0864&DestApp=IC2JCR
    Cited Times(WOS): https://www.webofscience.com/wos/woscc/full-record/WOS:000317478500001
    Cited Times(Scopus): http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=84876104810
    顯示於類別:[陳炯東] 期刊論文
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